Walnut-Check ELISAs
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Walnut-Check kits quantitatively detect walnuts in fresh & processed food products and surface swabs. The kits use a simple 3-step, 60 minute sandwich assay format and contain all the reagents required for the extraction and detection of walnut between 2 & 50 mg/kg (PPM); the LOD is <1 PPM of walnut. All allergen-specific reagents are ready to use and the kits are easily automated.
Select a Kit Size below for pricing (INCLUDES VAT) or contact us for multiple kit/standing order discounts.
See below for further product information.
The assay utilises polyclonal antibodies to detect walnut protein present in extracts of food-related samples.
The assay is a direct “sandwich” ELISA, designed to detect walnut proteins at very low levels in fresh, processed and heated food products as well as in environmental swabs.
The assay range is nominally between 2 & 50 ppm (mg/kg) walnut if the stated extraction ratio is used and the sample extract is not diluted. The range can be extended upwards by amending the extraction ratio, and/or diluting the sample extract, if required. range corresponds to ~0.3-7.5 ppm (mg/kg) walnut protein.
The Limit Of Detection (LOD) of the assay, 0.6 ppm (mg/kg) walnut, which corresponds to ~0.1 ppm (mg/kg) walnut protein.
See "How To Use" for an outline of the Sample Preparation and ELISA methodologies.
Or click here for the product information sheet.
Laboratory Sample Preparation
- Prepare Sample by grinding/chopping/blending until homogeneous.
- Add 1 part Test Portion to 20 parts Extraction Buffer 1X.
- Extract Incubate for 15 minutes @ 60°C Shaking every two minutes.
- Separate Centrifuge for 10 minutes.
- The sample extract can be used in several other "Allergen-Check" ELISAs.
ELISA Protocol
- Pipette 100µL Standards and Sample Extracts into wells.
- Mix. Incubate at room temperature for 20 minutes.
- Wash wells three times with Wash Solution.
- Pipette 100µL HRP-Conjugate into wells.
- Mix. Incubate at room temperature for 20 minutes.
- Wash wells three times with Wash Solution.
- Pipette 100µL TMB Substrate reagent into wells.
- Mix. Incubate at room temperature in the dark for 20 minutes.
- Pipette 100µL Acid Stop Solution into wells.
- Mix. Read wells at 450nm wavelength within 15 minutes.
- Calculate PPM Walnut results for all Samples.
