DON Test HPLC columns

  Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed.  DON has been implicated in moldy corn toxicosis of swine. 

DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm. Because of concerns about DON, the United States FDA has instituted advisory levels of 1 ppm for wheat products for human consumption, 5 ppm for grain products for most animal feeds and 10 ppm for grain products for cattle feed. 

The European Community has regulations for DON levels that range from less than 0.2 ppm in processed cereal-based foods for infants and young children to less than 1.75 ppm for unprocessed wheat.

Using monoclonal antibody-based affinity chromatography, DONtest™ HPLC is an HPLC-only test for the detection of DON using immunoaffinity columns. Performed in less than 10 minutes, DONtest HPLC can measure levels of DON as low as 0.10 ppm and as high as 5 ppm. 

An ideal cleanup step for any HPLC, DONtest HPLC has a long shelf life and provides precise numerical results in parts permillion levels. The preferred test of laboratories around the world, no other test comes close for speed, quantification, and specificity.

How it Works

How to Use

• Extract Sample

• Grind and weigh sample

• Add PEG and water to sample

• Filter

• Absorb and Elute

• Pass filtrate over DONtest HPLC affinity column

• Wash column

• Elute DON and collect in cuvette

• Measure

• Dry down eluate

• Resuspend in mobile phase

• Inject into HPLC

• Determine DON concentration

Using monoclonal antibody-based affinity chromatography, DONtest™WB is an HPLC-only test for the detection of DON using wide bore immunoaffinity columns. With a total volume of 3 mL, compared to the total of 1mL in VICAM’s regular columns, DONtest WB allows for the faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

DONtest WB is one of many wide bore columns available from VICAM.

How it Works

• Exclusive — Specifically for HPLC use
• Durable — Long shelf life
• Versatile — For use with a variety of samples
• Quick — 10 minutes to isolate toxin*
• Wide Range — Detects levels as low as 0.04 ppm and
  as high as 5 ppm
• Fast Flow — Faster results than narrow bore test

*excluding preparation and extraction

How to Use

• Extract Sample

• Grind and weigh sample

• Add water and shake

• Filter

• Absorb and Elute

• Pass filtrate over DONtest WB affinity column

• Wash column

• Elute DON and collect in a cuvette

• Dry eluate and reconstitute

• Measure

• Inject eluate into HPLC

• Determine DON concentration