CitriTest Columns

Citrinin, a toxin from a naturally occurring mold, contaminates rice, wheat, flour, barley, maize, rye, oats, peanuts, and fruit. It is also a widespread contaminant of red rice, which is not only a dietary staple in many Asian countries, but also a main ingredient in many other food products. It is also used in the popular herbal supplement red yeast rice extract which is used to reduce cholesterol levels.

Produced by Aspergillus ochraceus and Penicillium citrinum, citrinin has been known to cause kidney damage and mild liver damage in livestock and is suspected to cause kidney and liver damage in humans. Because of concerns over citrinin contamination, Japan has issued an advisory limit of 200 ppb and the European Union has a recommended limit of 100 ppb.

Using monoclonal antibody-based affinity chromatography, CitriTest™ HPLC is an HPLC-only test for the detection of citrinin using immunoaffinity columns. Performed in less than 10 minutes, CitriTest HPLC can measure levels of citrinin as low as 10 PPB and as high as 500 PPB.
An ideal cleanup step for any HPLC, CitriTest HPLC has a long shelf life and provides precise, accurate numerical results in parts per billion levels. The preferred test of laboratories around the world, no other test comes close for speed, quantification, and specificity.

How it Works

• Exclusive — Specifically for HPLC use
• Convenient — Uses same methods as other VICAM tests
• Durable — Long shelf life
• Quick — 15 minutes to isolate toxin*
• Wide Range — Detects levels as low as 10 ppb and
  as high as 500 ppb

*excluding preparation and extraction

How to Use

• Extract Sample

• Grind and weigh sample

• Add methanol/water mixture

• Filter

• Dilute and Filter

• Dilute extract

• Absorb and Elute

• Pass filtrate over CitriTest HPLC affinity column

• Wash column

• Elute citrinin and collect in cuvette

• Measure

• Inject into HPLC

• Determine citrinin concentration