Casein-Check (MILK/Caseins) ELISAs
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Casein-Check kits quantitatively detect caseins in fresh & processed food products and surface swabs. They use a simple, 3-step, 60 minute sandwich assay format and contain all the reagents required for the extraction and detection of skim milk powder (SMP) between 0.8 & 16.0 mg/kg (PPM); the LOD is <0.2 PPM of SMP. Most reagents are ready to use and the kits are easily automated.
Select a Kit Size below for pricing (INCLUDES VAT) or contact us for multiple kit/standing order discounts.
See below for further product information.
Skim Milk Powder (SMP) contains approximately 31% Casein by weight.
This assay utilises polyclonal antibodies to detect casein, as a marker for the presence of milk and caseins/caseinates, using a direct “sandwich” ELISA technique.
It is designed to detect caseins at low levels in food raw materials, fresh and lightly processed food products as well as in environmental swabs.
The assay range is nominally between ~0.25-5.0 mg/kg (PPM) casein, which corresponds to ~0.8-16.0 PPM SMP content if the stated extraction ratio is used and the sample is not diluted. The range can be extended upwards by amending the extraction ratio and/or diluting the sample extract if required.
The Limit Of Detection (LOD) of the assay is ~0.05 mg/kg (PPM) casein, which corresponds to <0.2PPM SMP content.
See "How To Use" for an outline of the Sample Preparation and ELISA methodologies.
Or click here for the product information sheet.
Laboratory Sample Preparation
- Prepare Sample by grinding/chopping/blending until homogeneous.
- Add 1 part Test Portion to 20 parts Extraction Buffer 1X.
- Extract Incubate for 15 minutes @ 60oC Shaking every two minutes.
- Separate Centrifuge for 10 minutes.
ELISA Protocol
- Pipette 100µL Diluted Standards and 100µL Sample Extracts into wells.
- Mix. Incubate at room temperature for 20 minutes.
- Wash wells three times with Wash Solution.
- Pipette 100µL HRP-Conjugate into wells.
- Mix. Incubate at room temperature for 20 minutes.
- Wash wells three times with Wash Solution.
- Pipette 100µL TMB Substrate reagent into wells.
- Mix. Incubate at room temperature in the dark for 20 minutes.
- Pipette 100µL Acid Stop Solution 1 into wells.
- Mix. Read wells at 450nm wavelength within 15 minutes.
- Calculate PPM SMP results for all Samples.
