AflaTest Columns

Aflatoxins are Group 1 human carcinogens; the United States federal government action level for total aflatoxin in food for human consumption or in dairy cow's feed is 20 ppb. In milk for human consumption the level is 0.5 ppb. Levels up to 300 ppb are allowed in certain circumstances for non-dairy animal feed. Similar regulations exist in the EU and worldwide.

AflaTest^® from VICAM is the trusted aflatoxin test that produces accurate, precise numerical results. Using monoclonal antibody-based affinity chromatography, AflaTest can isolate aflatoxins B₁, B₂, G₁, and G₂ from feeds, food, grains, and nuts, and M₁ from dairy products.

AOAC and FGIS approved, AflaTest is safe and simple. It can be performed in less than 10 minutes and requires no special skills. Results may be obtained using HPLC or a fluorometer.  With AflaTest, you have the best of all worlds: sensitivity, simplicity, and speed—quick tests for parts per billion levels. In fact, no other test comes close for speed, quantification, and economy.

AflaTest P from VICAM is the trusted total aflatoxin test that produces accurate, precise numerical results for aflatoxins B1, B2, G1, and G2 in animal feeds, food, grains, and nuts.  

 AflaTest uses monoclonal antibody-based affinity chromatography  and can isolate total aflatoxins (B1, B2, G1, and G2) from feeds, food, grains, and nuts quickly, economically and effectively.

• Convenient – For use with fluorometer or HPLC
• Comprehensive – Total readings for all aflatoxins
• Durable – Long shelf life; requires no refrigeration
• Versatile – For use with a variety of sample types
• Quick – Less than 10 minutes to isolate toxin*
• Wide Range – Detects levels as high as 300 ppb and as low as 0.1 ppb**
• Safe – Requires less toxic materials than other methods

          *excludes preparation and extraction

           ** 1 ppb for grain and nuts

How to Use

• Extract Sample
• Grind and weigh sample
• Add salt and methanol/water mixture ; filter
• Dilute extract; filter
• Pass filtrate over AflaTest affinity column; wash column
• Elute aflatoxins with methanol and collect in cuvette
• Add developer and place cuvette into a calibrated fluorometer,        OR:
• Inject eluate into HPLC and determine aflatoxin concentration

Using monoclonal antibody-based affinity chromatography, AflaTest® WB is an HPLC-only test for the detection of aflatoxins using wide bore immunoaffinity columns to improve flow rates.  

With a total volume of 3mL, compared to the total of 1mL in VICAM’s standard columns, AflaTest WB allows for the faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

AflaTest WB is one of many wide bore columns available from Vicam and offered by DIL to customers in the UK & Ireland:

• Exclusive – Specifically for HPLC use
• Comprehensive – Total readings for all aflatoxins
• Durable – Long shelf life; requires no refrigeration
• Versatile – For use with a variety of samples
• Quick –15 minutes to isolate toxin*
• Wide Range – Detects levels as high as 100 ppb and as low as 0.03 ppb for aflatoxin B₁
• Fast Flow – Passes more volume over the column

*excluding preparation and extraction

How to Use

• Extract Sample

• Grind and weigh sample

• Add salt and methanol/water mixture

• Filter

• Dilute and Filter

• Dilute extract

• Filter

• Absorb and Elute

• Pass filtrate over AflaTest WB affinity column

• Wash column

• Elute aflatoxins with methanol and collect in cuvette

• Measure

• Dilute and inject eluate into HPLC

• Determine aflatoxin concentration

VICAM’s AflaTest®WB SR columns use immunoaffinity chromatography to selectively isolate aflatoxins B1, B2, G1, G2, and M1 for HPLC analysis.

VICAM’s AflaTest®WB SR column uses immunoaffinity chromatography to selectively isolate aflatoxins B1, B2, G1, G2, and M1 for HPLC analysis.
The SR test kit’s fast-flow widebore columns are specially designed to maximise aflatoxin G2 recovery and accelerate sample throughput.

AflaTestWB SR is the latest additon to VICAM’s growing line of wide-bore column HPLC-only test kits.

• Powerful – Designed exclusively for laboratory use
• Enhanced Recovery – Improved aflatoxin G2 recovery
• Comprehensive – Determines individual aflatoxin levels (B₁, B₂, G₁, G₂, and M₁ ) and total (B₁, B₂, G₁, and G₂) concentration
• Durable – Long shelf life; requires no refrigeration
• Versatile – For use with a variety of samples
• Quick – 15 minutes to isolate toxin*
• Wide Range – Detects total aflatoxin levels as high as 500 ng and as low 0.005 ng for total aflatoxin
• Fast Flow – Passes more volume over the column

*excluding preparation and extraction

How to Use

• Extract Sample

• Grind and weigh sample

• Add salt and methanol/water mixture

• Filter

• Dilute and Filter

• Dilute extract

• Filter

• Absorb and Elute

• Pass filtrate over AflaTest WB SR affinity column

• Wash column

• Elute aflatoxins with methanol and collect in cuvette

• Measure

• Dilute and inject eluate into HPLC

• Determine aflatoxin concentration