AflaTest B & Afla M1 columns

Aflatoxins, produced in the environment by mould species, are Group 1 human carcinogens. They comprise four separate aflatoxins: B₁, B₂, G₁, and G₂ (so called total aflatoxins); however, in some cases only B₁ is regulated; for example, within the European Community B₁ is regulated at the 2ppb level.

Afla B™ from VICAM is the trusted aflatoxin B test that produces accurate and precise numerical results. Using monoclonal antibody-based affinity chromatography, Afla B can isolate aflatoxins B₁ and B₂ from feeds, food, grains, and nuts for either fluorometric or HPLC detection.

Whereas aflatoxin B₁ can be separated out and measured alone by HPLC, in fluorometric measurements, the total of B₁ and B₂ is reported. It is estimated that B₁ accounts for 94% of total B₁ and B₂ in corn and 82% in peanuts.

When mammals ingest aflatoxins in food, the derivative aflatoxin M₁ is produced. Because it is a potent human carcinogen, the detection of M₁ is critical, not least because M₁ is expressed in milk, a major source of nutrition for infants, children, and adults. Throughout the world, there are significant regulations on the presence of M₁ in dairy products. VICAM’s Afla M₁™ HPLC is a fast flow column that delivers high recovery rates, from 75% to 95%, depending on the sample type.

Afla B testing is safe & simple and can be performed in less than 10 minutes, requiring no special skills. With Afla B, you have the best of all worlds: sensitivity, simplicity, and speed – quick tests for parts per billion or parts per trillion levels. In fact, no other test comes close for speed, quantitation and economy.

Afla B™ from VICAM is the trusted aflatoxin B test that produces precise numerical results. Using monoclonal antibody-based affinity chromatography, Afla B can isolate aflatoxins B1 and B2 from feeds, food, grains, and nuts via fluorometric or HPLC detection.

Aflatoxin B1 can be singled out and measured uniquely by HPLC, but in fluorometric measurements, total B1 and B2 is reported.

It is estimated that B1 accounts for 94% of total B1 and B2 in corn and for 82% in peanuts.

• Convenient – For use with HPLC or fluorometer
• Durable – Long shelf life; requires no refrigeration
• Versatile – For use with a variety of samples
• Quick – Less than 10 minutes to isolate toxin*
• Wide Range – Detects levels as low as 1 ppb (fluorometer) and 50 ppt (HPLC) and as high as 300 ppb
• Safe – Requires less toxic materials than other methods

          * excludes preparation and extraction

How to Use

• Extract Sample
• Grind and weigh sample
• Add salt and methanol/water mixture
• Filter
• Dilute and Filter
• Dilute extract
• Filter
• Absorb and Elute
• Pass filtrate over Afla B affinity column
• Wash column with water
• Elute aflatoxins with methanol and collect in a cuvette
• Measure
• Add developer and place cuvette into a calibrated fluorometer
• Or
• Inject eluate into HPLC
• Determine aflatoxin concentration

Using monoclonal antibody-based affinity chromatography, Afla M1 HPLC is an HPLC-only test for the detection of aflatoxin M1 at very low levels. Performed in less than 30 minutes, Afla M1 HPLC can measure levels of Afla M1 as low as 10 ppt and as high as 3 ppb.

An ideal cleanup step for any HPLC method, Afla M1 HPLC has a long, room temperature stored, shelf life and provides accurate, precise numerical results in parts per billion or parts per trillion levels. The preferred test of laboratories around the world, no other test comes close for speed, quantification, and specificity.

• Exclusive – Specifically for HPLC use
• Durable – Long shelf life; requires no refrigeration
• Versatile - Can be used with a variety of milk sample types
• Quick – Sample ready fir analysis in less than 30 minutes
• Wide Range - Detects levels as low as 10 ppt and as high as 3 ppb
• Safe – Requires less toxic materials than other methods

How to Use

• Prepare Sample
• Centrifuge
• Absorb and Elute
• Pass sample over Afla M₁ HPLC affinity column
• Wash column
• Elute aflatoxin M₁ and collect in cuvette
• Measure
• Inject eluate into HPLC
• Determine aflatoxin M₁ concentration